10% Buffered Neutral Formalin

10% B.N.F Solution – N.C.C

10% Buffered Neutral Formalin Solution

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Intended use

10% Buffered Neutral Formalin is intended as a general purpose fixative for all tissues to be examined by light microscopy . It is useful for surgical tissues and necropsy specimens.

 

General information

10% Buffered Neutral Formalin is a non-coagulative additive fixative. It is the standard fixative used in most laboratories and it produces the set of histologic artifacts that Pathologist are trained to interpret professionally.10% Neutral Buffered Formalin penetrates quickly, but fixes slowly . When used as a primary fixative and followed by secondary fixation by alcohol , It produces a marvelous set of consistent histologic artifacts and results. Our formulation is enhanced by capacity that optimizes histologic results by light microscopy and immunohistochemistry.

                                                                                                                               

                                                                                                                Fixation procedure

10% Buffered Neutral Formalin is a a non-coagulative additive fixative , It is intended to be used as the standard fixative in the histology laboratory. The buffering capacity of our 10% Neutral Buffered Formalin enhances staining by H&E and immunohistochemistry.

1. The biopsies or tissues should be added directly to the 10% Neutral Buffered Formalin. No other dilution or addition of other agents is necessary before use.

2. Small biopsies, such as bone marrow biopsies should be fixed at least 3 hours prior to processing. Large tissues, such as tissue blocks from lymph nodes or spleen or breast or colon, are best fixed 10 to 12 hours , although fixation for 4 to 6 hours is often sufficient . Over fixation is not a problem, however , tissues should generally not be fixed longer than 1 to 2 weeks.

3. No washing to tissues after fixation is necessary.

4. The fixed tissues should be processed by the standard processing schedules that may vary from

1 hour to 12 hours.

Histopathology Tissue Fixation with 10% Buffered Neutral Formalin N.C.C

 

Purpose

Formalin is a good routine fixative. This solution is hypertonic in buffer ions and a pH of 6.8. specimens may be retained in 10% formalin indefinitely. But over fixation ( 2 weeks) may impair suitability for immunohistochemistry.

                                                                                                                             

                                                                                                                     Procedure

Submerge tissue samples in pre-filled, formalin- resistant container that can be tightly closed . Add 10% formalin to achieve perfect ratio for immersion fixation (tissue : fixative) 1:10,but 1:5 may be acceptable.

 

 

Fixation time depends on size of specimen but it should be within 20 minutes. Label with formaldehyde Warning label (Yellow).

 

Safety

 

Work in well-ventilated area, wear goggles , nitrile gloves, and a lab coat. Call EHS for large spills , Use Spill -X-FP for small spills. Formaldehyde is a severe eye and skin irritant and sensitizes by skin and respiratory contact. It is toxic by ingestion and inhalation. The respiratory system is the target organ. It is Carcinogenic and Corrosive.